The effect of miR-539 regulating TRIAP1 on the apoptosis, proliferation, migration and invasion of osteosarcoma cells

Abstract Objective The purpose of this study is to explore the effect miRNA-539 on osteosarcoma (OS) and underlying mechanism, so as find a new method for early diagnosis treatment osteosarcoma. Method mimics was transfected into cells 143b MG-63 upregulated expression miR-539. QT-PCR used detect transfection efficacy. CCK-8 proliferation flow cytometry apoptosis MG-63. Wound-healing test Transwell were migration invasion ability cells. TRIAP1 found be potential target gene by online bioinformatics software level in overexpressing detected qT-PCR. Western blot its downstream genes (p53, p21, apaf1 caspase9) MG63 with miR-539 or mimics-NC. A model subcutaneously transplanted nude mice constructed observe growth rate vivo. Results After MG63, level, ability, significantly lower than that control group, higher group ( P < 0.01). dual luciferase reporter confirmed miR-539, proved 0.01).The western showed targeted when downregulated. In vivo, slower Conclusions MiRNA-539 may inhibit cell proliferation, promote targeting TRIAP1.